Agenda - Mechanobiology of the Nucleus

8—9 am: Breakfast

9 am—12:30 pm: Session 2: Mechanics of the cell nucleus and its connection to the Cytoskeleton
Chair: Dan Starr

9—9:30 am: Ohad Medalia, University of Zurich - "Structural Insight into nuclear lamina-chromatin interactions"
9:30—10 am: Andrew Stephens, U Mass Amherst - “Transcription activity drives coherent chromatin motion to cause nuclear bleb formation, stabilization, and rupture”
10—10:15 am: Short talk: Rashmi Budhathoki, University of Denver, “A transition to perinuclear non-centrosomal MT networks directs dispersion of nuclei during epithelial morphogenesis”
10:15—10:30 am: Short talk: Daria Amiad Pavlov, Technion IIT “Nuclear strain and fragility in beating cardiomyocytes - implications for laminopathies”
10:30—11 am: Coffee break
11—11:30 am: Zeinab Jahed, UCSD - “Nanoscale biomaterials for probing and modulating nuclear mechanics”
11:30 am—12 pm: Gregg Gunderson, Columbia University Medical School -
“The role of FHOD3-LINC complex interaction in sarcomere formation and mechanical integrity”
12:00-12:30 pm Megan King / Sandra Sandria, Yale University “Disrupting nuclear mechanosensing short circuits feed-forward signaling that drives organ fibrosis”

12:40—2 pm: Lunch and Career Roundtable discussion topics

Table 1 - Postdoc planning: finding labs and other considerations (Verena Ruprecht/Philipp Niethammer)
Table 2 - Expert guide to mentoring - the mentor map (Megan King)
Table 3 - Starting a lab/transitioning from postdoc to PI (Kate Miroshnikova/Hawa Racine Thiam)
Table 4 - Staying innovative throughout your career (Jan Lammerding/Sara Wickström)

2—3:30 pm: Free time

3:30—7:15 pm: Session 3: The role of the nucleus in cellular mechanotransduction
Chair: Kate Miroshnikova

3:30—4 pm: Pere Roca-Cusachs, IBEC Barcelona, Spain - “Transducing - and shielding - mechanical signals from integrins to the nucleus”
4—4:30 pm: Jan Lammerding/Julien Morival, Cornell University - “New insights into ultra-rapid nuclear mechanotransduction”
4:30—4:45 pm: Short talk: Haisha Liang, Yale School of Medicine, USA “Live imaging in mice links nuclear dynamics to skin maturation”
4:45—5:15 pm: Break
5:15—5:45 pm: Sevan Hopyan, SickKids Toronto “Force transduction to nuclei in the mammalian limb bud”
5:45—6 pm: Short talk: Abira Ganguly, Washington University, USA “The ALT-LINC complex mediates cellular responses to matrix mechanics”
6—6:15 pm: Short talk: Navraj Lally, University of California, San Francisco “Isoform-specific disruption of nesprin-1G perturbs nuclear homeostasis and cardiac function”
6:15—6:45 pm: Philipp Niethammer, Memorial Sloan Kettering Cancer Center - “Nuclear mechanotransduction in wound detection and tissue adaptation to mechanical stress in zebrafish”
6:45—7:15 pm: Poster Flash Talks

7:15—8:30 pm: Dinner

8:30—10 pm: Poster Session I & Social

8—9 am: Breakfast

9 am—12:30 pm: Session 4: Mechanobiology of nuclear size, the cell cycle, and stress adaptation
Chair: Kohta Ikegami

9—9:30 am: Jorge Ferreira, University of Porto, Portugal - “Mechanical priming of the nucleus is required for mitosis”
9:30—10 am: Dennis Discher, University of Pennsylvania “From mechanoregulated cancer mutations to lamin-eng’ed tumoricidal macrophages for acquired immunity”
10—10:15 am: Short talk: Yannick Gachet, Université de Toulouse, France. “Mechanisms Controlling Mitotic Robustness under Cell Confinement”
10:15—10:30 am: Short Talk: Mehdi Hamouda, Massachusetts Institute of Technology, Cambridge, USA “Investigating the role of nuclear mechanotransduction in trisomy 21 cardiac phenotypes”
10:30—11 am: Coffee break and Group Picture
11—11:30 am: Gabriel Neurohr, ETH Zurich - "Nuclear mechanisms of cell size induced senescence"
11:30—11:45 am: Short talk: Kerri Davidson, Yale School of Medicine “Unveiling the role of the integral nuclear envelope protein, MAN1, in extracellular matrix homeostasis”
11:45 am—12 pm: Short talk: Zaza Gelashvili, Memorial Sloan Kettering “Perivascular macrophages convert physical wound signals into rapid vascular responses”
12—12:30 pm: Verena Ruprecht, CRG Barcelona, Spain “Nucleus mechano-metabolism as a regulator of DNA damage and cell fitness”
12:30-12:45 pm Short Talk: Yi Wei, "Application of Large Area Mapping and High-Resolution Correlative Imaging with AFM for Automated Structural and Nanomechanical Analysis in Tissues and Cells", Bruker

12:45—2 pm: Lunch and Meet the Experts

Table 1 - Bioinformatics, Computational Modeling, and Artificial Intelligence (Vivek Shenoy, Rachel McCord)
Table 2 - Mechanical Measurements of Cells & Nuclei (Drew Stephens, Yi Wei [Bruker], Frede Catala [Impetux])
Table 3 - Imaging and Determining Nuclear Molecular Structures (Ohad Medalia, Martin Beck)

2—3:30 pm: Free time

3:30—7 pm: Session 5: Mechanical force control of nuclear integrity, cell state, and genomic stability
Chair: Sara Wickström

3:30—4 pm: Martin Beck, Max Planck Institute of Biophysics, Frankfurt, Germany - “How human immunodeficiency virus enters the nucleus”
4—4:30 pm: Abby Buchwalter, UCSF "Mapping sequence – folding – function relationships with deep mutational scanning of the nuclear lamina"
4:30—4:45 pm: Short talk: Léa Mayeur, University Paris-Saclay, France “Mechanical memory is encoded by nuclear membrane tension-regulated H3K27 methylation dynamics”

4:45—5:15 pm: Break

5:15—5:45 pm: Christian Zierhut, Institute of Cancer Research, London, UK "cGAS-STING cross-talk controls cancer-associated fibroblast polarisation and ECM production”
5:45—6:15 pm: Kate Miroshnikova, Max Planck Institute for Molecular Biomedicine, Muenster, Germany “Nuclear mechanotransduction in genome integrity & malignant transformation”
6:15—6:30 pm: Short talk: Helen McNeill, Washington University, USA “The role of the nuclear envelope protein 1 (NEMP1) in nuclear envelope rupture and repair”
6:30—7 pm: Poster Flash Talks

7—8:15 pm: Dinner

8:30—10 pm: Poster Session II & Social

8—9 am: Breakfast

9:15 am—12:30 pm: Session 6: Biophysical Mechanisms and Modeling in Nuclear Mechanobiology
Chair: Verena Ruprecht

9:15—9:45 am: Rachel McCord, University of Tennessee "3D Genome Resilience, Remodeling, and Remembering: the effect of physical stresses to the nucleus on human genome architecture and cell fate"
9:45—10:15 am: Vivek Shenoy, University of Pennsylvania “Sub-Diffraction Chromatin Domains: Architecture, Regulation, and Functional Roles in Nuclear Organization”
10:15—10:30 am: Short Talk: Nina Nikitina, Boise State University “Nuclear motion enables cell response to small accelerations independent of matrix deformations”
10:30-10:45 am Short Talk: Frederic Català Castro, "SENSOCELL: Deciphering cell mechanobiology through optical tweezer force spectroscopy", IMPETUX

10:45—11 am: Coffee break

11 am—12 pm: Business Meeting

12:15—2 pm: Lunch and Career Roundtable Discussion

2—3 pm: Free time

3:30—6:30 pm: Session 7: When nuclear mechanobiology goes awry - the nucleus and disease
Chair: Jan Lammerding

3:30—4 pm: Valerie Weaver/Mary-Kate Hayward, University of California, USA “Fibrosis promotes tumor mutagenesis and drives malignancy through myeloid-lipid peroxidation”
4—4:30 pm: Kohta Ikegami, Cincinnati Children’s Hospital, “Nuclear membrane rupture and associated repair in Lamin A/C-related cardiomyopathy.”
4:30—4:45 pm: Short talk: Daniel A. Starr, University of California, USA. “Muscular dystrophy-associated lamin mutations disrupt cytoplasmic macromolecular crowding via a nucleolar-ribosome axis”
4:45—5:15 pm: Coffee break
5:15—5:45 pm: Matthew Stroud, King's College London, London, UK “Revealing roles of the nuclear envelope in cardiovascular development and disease”
5:45—6:00 pm: Short talk: Yin Loon Lee, A*STAR, Singapore “Investigating the role of nuclear-cytoskeletal coupling in liver fibrosis”
6:00-6:30 pm Hawa Racine Thiam, Stanford University “The biophysical and non-genetic role(s) of chromatin in immune cell functions - Learning from NETosis”

7:15—8:30 pm: Dinner

8:30—10:00 pm: Farewell Reception